This wide-ranging collection offers more than a dozen expert contributions on the field of molecular biology and current techniques. In part one, contributions address selected applications of molecular cloning in medicine and biology. Topics examined include subcloning and expression of functional human cathepsin B and K in E. coli. Part two considers bacterial artificial chromosomes, and focuses on the recombineering of BAC DNA for the generation of transgenic mice. Part three considers advanced techniques in gel electrophoresis. Topics examined include an analysis of chromosomal replication progression by gel electrophoresis. Part four considers the basics and principles of gel electrophoresis, and includes studies of quantitative analysis of electrophoresis data. Part five focuses on RNA processing, and includes a study of chromatic remodelling and RNA processing. Part six, finally, examines genetic transformation, and focuses on genetic transformation of Triticeae cereals for molecular farming. This comprehensive collection will be essential reading for students and researchers in molecular biology seeking current perspectives on the field.
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